Articles

Preliminary report of a novel formulation of clinical-grade, fully matured, tumor-associated peptide-loaded dendritic cells for cancer immunotherapy

We developed and clinically test a new method to manufacture mature dendritic cells for autologous cell therapy of solid and hematological malignancies.

Authors: Leonardo Mirandola, Maurizio Chiriva-Internati, Robert Bresalier, Francesco M. Marincola, Jose A. Figueroa and Scott Dahlbeck

Forming effective relationships between academia and the medical devices industry with a focus on launching a smart heart valve prosthesis for pediatric patients

This article presents examples of how to utilize the research output, to initiate academia-industry interaction, with the ultimate task of launching a new product: a smart heart valve prosthesis for pediatric ...

Authors: Albert R. Liberski, Jonathan T. Butcher, Zbigniew Nawrat, Tal Golesworthy and Dena Ahmed Al Thani

Proteome analysis of Mycoplasma fermentans cultured under aerobic and anaerobic conditions

Mycoplasmas are ubiquitous pathogens found not only in humans but also in animals, plants, insects and soil. Though they usually grow better in an aerobic environment, mycoplasmas are also facultative anaerobi...

Authors: Francesca Benedetti, Selvi Krishnan, Fiorenza Cocchi, Hervé Tettelin, Robert C. Gallo, Davide Zella and Sabrina Curreli

Differences and overlap in plasma protein expression during colorectal cancer progression

Colorectal cancer (CRC) is the third most common cancer worldwide, and its incidence is expected to increase to over 2.2 million new cases in 2030. Stage II CRC is classified as localized disease, while stage ...

Authors: Matilda Holm, Sakari Joenväärä, Mayank Saraswat, Tiialotta Tohmola, Ari Ristimäki, Risto Renkonen and Caj Haglund

Anglemetry of neural axis cell differentiation genes by structural pressurotopy of DNA loop strand segment tropy in reference to tissue macro-compliance

Even as the eukaryotic stranded DNA is known to heterochromatinize at the nuclear envelope in response to mechanical strain, the precise mechanistic basis for alterations in chromatin gene transcription in dif...

Authors: Hemant Sarin

Comorbid brain disorders associated with diabetes: therapeutic potentials of prebiotics, probiotics and herbal drugs

Prevalence of diabetes and comorbid brain disorders is increasing worldwide particularly in low- and middle-income countries. Diabetes and its complications impose substantial economic costs on the developing ...

Authors: Ajit Kumar Thakur, Sakshi Tyagi and Nikhila Shekhar

Simplified assay for enrichment of primed human Th17 and Tc17 lymphocytes from peripheral blood

Interleukin-17A (IL-17A) is a potent pro-inflammatory cytokine that has been implicated in the pathogenesis of various autoimmune diseases. The production of IL-17A is commonly associated with subsets of CD4+ ...

Authors: Pradeep K. Dagur, Elena Stansky, Ankit Saxena, Angélique Biancotto and John Philip McCoy Jr

Precision medicine in relapsed or refractory pediatric solid tumors: a collaborative Spanish initiative

Understanding pediatric cancer biology is a huge challenge in continuous development that is currently being implemented into the clinical practice thanks to the new high throughput technologies integrated by ...

Authors: Pablo Gargallo, Jaime Font de Mora, Pablo Berlanga, Inés Calabria, Margarita Llavador, Laia Pedrola, Joaquín Panadero, Sandra Dolz, Ángel Zúñiga, Juan Silvestre Oltra, Paloma Escobar, Yania Yáñez, María José Aparisi, Marina Martinez-Matilla, Vanessa Segura, Carlos Esquembre…

Endogenous fluorescence of hemosiderin in endometriosis to improve clinical detection

Endometriosis impacts 6–10% of all reproductive- age women. Studies have shown the more effectively endometriosis is removed, the better the patient outcomes for pain reduction and fertility (2, 3). Hemosideri...

Authors: Andrew G. Cabe, Arnold D. Estrada, Taylor Hoyt, Xiao Yang, Scott Jenney, Philip T. Valente, Bryan Cox, Jessica E. McLaughlin, Randal D. Robinson, Thomas E. Milner and Marc D. Feldman

First-line antiretroviral treatment and virological response among HIV adult patients at an accredited HIV care center, Cameroon

Viral load (VL) monitoring in the clinical follow-up of HIV patients to detect treatment failure is not routinely offered in Cameroon. The study was designed to characterize virological response on antiretrovi...

Authors: Juste Patient Mbébi Enoné, Calixte Ida Penda, Serge Bruno Ebong, Madeleine Mbangue, Samuel Honoré Mandengué and Carole Else Eboumbou Moukoko

Performance and immune characteristics of bronchoalveolar lavage by research bronchoscopy in pulmonary tuberculosis and other lung diseases in the Western Cape, South Africa

Tuberculosis (TB) remains a debilitating, deadly disease that warrants innovative research tools to fully understand the pathogenesis and host immune responses, particularly at the site of infection and diseas...

Authors: Carly Young, Petri Ahlers, Andriette M. Hiemstra, Andre G. Loxton, Andrea Gutschmidt, Stephanus T. Malherbe, Gerhard Walzl and Nelita Du Plessis

Label-free serum proteomics and multivariate data analysis identifies biomarkers and expression trends that differentiate Intraductal papillary mucinous neoplasia from pancreatic adenocarcinoma and healthy controls

Intraductal Papillary Mucinous Neoplasia (IPMN) are potentially malignant cystic tumors of the pancreas. IPMN can progress from low to moderate to high grade dysplasia and further to IPMN associated carcinoma....

Authors: Mayank Saraswat, Heini Nieminen, Sakari Joenvaara, Tiialotta Tohmola, Hanna Seppänen, Ari Ristimäki, Caj Haglund and Risto Renkonen

Prunus armeniaca effects on expression of genes related to apoptosis in human breast cancer cells

Apoptosis inhibition depends on the balance between expression of some regulatory genes including Bax and c-FLIP, despite breast cancer has long been associated with disturbances in apoptosis regulation. The obje...

Authors: Esmaeil Mahmoudi, Mahdieh Abolfathi, Naiemeh Hassanzadeh, Yaser Eshaghi Milasi, Mina Dehghani-Samani, Mansoor Khaledi, Hossein Kerdarian, Mina Najafipour and Asghar Arshi

Skewed CD39/CD73/adenosine pathway in B cells is associated with innate immune hyperactivation in chronic HIV-1 infection

The CD39/CD73/adenosine suppression pathway correlates with disease progression in patients with chronic human immunodeficiency virus 1 (HIV-1) infection; however, the relationships between this pathway in B c...

Authors: Wen-Xian Chang, Hui-Huang Huang, Lei Huang, Ji-Jing Shi, Yan-Mei Jiao, Chao Zhang, Lei Jin, Tao Yang, Ming Shi, Bo Tu, Zhe Xu, Tian-Jun Jiang, Fu-Sheng Wang and Ji-Yuan Zhang

Sirtuins and diabetes: optimizing the sweetness in the blood

Diabetes Mellitus (DM) is a chronic disease characterized by elevated levels of glucose in the blood. With time it becomes uncontrollable and invites other complex metabolic diseases. The propensity of the peo...

Authors: Abhinav Kanwal and Liston Augustine Dsouza

Biomarkers in immune checkpoint inhibition therapy for cancer patients: what is the role of lymphocyte subsets and PD1/PD-L1?

Although durable clinical responses are achieved in a significant number of patients given Immune checkpoint inhibitors (ICI), like anti-CTLA-4 and anti-PD-1 inhibitors, some of the cancers have shown little o...

Authors: Puneet Singh, Paul de Souza, Kieran F. Scott, Bruce M. Hall, Nirupama D. Verma, Therese M. Becker, James W. T. Toh, Mila Sajinovic and Kevin J. Spring

Oncogenic role of dysregulated leptin signaling in the pathogenesis of ovarian cancer

Leptin, a pleiotropic hormone is produced by adipose tissues and plays a critical role in various biological functions. Leptin mediates its function through interaction with specific receptors. The leptin-rece...

Authors: Iman W. Achkar, Ajaz A. Bhat, Maria Zafar, Madiha Abbas, Omar S. Khan, Shilpa Kuttikrishnan, Kirti S. Prabhu and Shahab Uddin

IKAROS expression profiles characterize different autoimmune diseases

Autoimmune diseases are syndromes characterized by an immune response against self-antigens. They are complex pathologies associated with a variety of genetic determinants, but these genes cannot fully explain...

Authors: Leydy Katherin Duque Suárez, Gerardo Quintana López, Paola Ximena Coral Alvarado, Paul Alejandro Méndez Patarroyo, Helena Groot deRestrepo and Valeriano López-Segura

The comparative effects of high fat diet or disturbed blood flow on glycocalyx integrity and vascular inflammation

Endothelial surface glycocalyx shedding plays a role in endothelial dysfunction and increases vessel wall permeability, which can lead to inflammation and atherogenesis. We sought to elucidate whether a high f...

Authors: Ronodeep Mitra, Ju Qiao, Sudharsan Madhavan, Gerard L. O’Neil, Bailey Ritchie, Praveen Kulkarni, Srinivas Sridhar, Anne L. van de Ven, Erica M. Cherry Kemmerling, Craig Ferris, James A. Hamilton and Eno E. Ebong

Verification of foetal Down syndrome biomarker proteins in maternal plasma and applications in prenatal screening for Down syndrome

Down Syndrome (DS) has a very high morbidity, according to statistics, the incidence rate of DS is as high as 1:700 among the new born babies. At present, there are still no effective prevention or treatment m...

Authors: Weiguo Sui, Qing Gan, Wei Wei Gong, Xiaolian Wei, Minglin Ou, Donge Tang, Huanyun Jing, Hua Lin, Yue Zhang and Yong Dai

Notch pathway connections in primary leukaemia samples of limited size

The Notch pathway combined with other signalling molecules acts specifically for the development of each blood cell type and differentiation stage. A causative role of Notch dysfunction in leukaemia developmen...

Authors: Luka Horvat, Josipa Skelin, Biljana Jelić Puškarić, Isidoro Feliciello, Darko Heckel, Josip Madunić, Ika Kardum-Skelin, Maja Matulić, Delfa Radić-Krišto and Mariastefania Antica

Phase I clinical trial results of aceneuramic acid for GNE myopathy in Japan

GNE myopathy (distal myopathy with rimmed vacuoles) is a rare intractable muscle disease caused by the mutations in GNE gene, with no therapeutic agents at present. The mutations in GNE (UDP-N-acetylglucosamine 2...

Authors: Naoki Suzuki, Masaaki Kato, Hitoshi Warita, Rumiko Izumi, Maki Tateyama, Hiroshi Kuroda, Ryuta Asada, Akifumi Suzuki, Takuhiro Yamaguchi, Ichizo Nishino and Masashi Aoki

Retrospective analysis to evaluate the efficacy and the safety of Bevacizumab in the treatment of recurrent malignant gliomas

There is no consensus therapy recommended for recurrent malignant gliomas (MGs). In 2009, Bevacizumab (BEV) was approved by the FDA as single-agent for recurrent glioblastoma (GBM). The aim of this retrospecti...

Authors: Arsela Prelaj, Sara Elena Rebuzzi, Maurizio Salvati, Silvia Pecorari, Chiara Pozzi, Carmela Fusto, Carla Ferrara, Silverio Tomao and Vincenzo Bianco

Common genetic variants in the sex hormone-binding globulin (SHBG) gene in idiopathic recurrent pregnancy loss: a case control study

A role for sex hormone-binding globulin (SHBG) in determining the pregnancy outcome was evidenced by the rise in SHBG levels during pregnancy linked with favorable pregnancy, while reduction in SHBG levels and...

Authors: Mariam Dendana, Ramzi R. Finan, Mariam Al-Mutawa and Wassim Y. Almawi

The role of free endosomal epitopes in the mechanisms of amelioration and flares of rheumatoid arthritis-associated conditions: pregnancy and infective hepatitis

It is known that rheumatoid symptoms improve in pregnancy and in patients who develop infective jaundice. The mechanism of amelioration might involve the direct interaction of free endosomal self-epitopes that...

Authors: Innocenzo Caruso, Enzo Massimo Caruso, Salvatore Santandrea, Franco Montrone, Piercarlo Sarzi Puttini and Marco Cazzola

B-cell differentiation is pressuromodulated as determined by pressuromodulation mapping: Part I, cell differentiation

The episodic sub-episode block sums split-integrated weighted average-averaged gene overexpression tropy quotient (esebssiwaagoT_Q) is a measure of the 5′ → 3′ reading direction intergene distance tropy that needs...

Authors: Hemant Sarin

B-cell antibody class switchings are pressuromodulated events: Part II, gene recombination

The esebssiwaagoT_Q method is applicable for the of study cell gene recombination events as the esebssiwaagoT_Q is a measure of the intracellular pressure required to establish a horizontal reading frame for alignm...

Authors: Hemant Sarin

Recapitulation of the anti-Idiotype antibodies as vaccine candidate

The foreign antigen that enters into the body is detected by the immune system through antigen presenting cells leading to the production of antibodies. The use of anti-idiotype antibodies is a new way to exci...

Authors: Ahsan Naveed, Sajjad Ur Rahman, Muhammad Imran Arshad and Bilal Aslam

Treating multiple antiaging pathways improves health markers in open label clinical study

Research indicates that aging and health are affected by hundreds of biochemical pathways. Our hypothesis is that a multipath intervention strategy directed at multiple aging pathways may promote overall healt...

Authors: Bryant Villeponteau and Yvonne L. Still

A Solanum incanum extract (SR-T100) regresses vulvar condyloma acuminatum and induces distinct autophagic and apoptotic responses in different types of HPV-infected cells

The Solanum species have been used for the treatment of warts, tumor and cancer in folk medicine. The S. incanum extract is an important traditional Chinese medicine in Taiwan since 1973. The purpose of the prese...

Authors: Yeong-Chang Chen, Meng-Ru Shen, Ai-Li Shiau, Hamm-Ming Sheu, Yu-Lin Liang and Keng-Fu Hsu

A practical prognostic lncRNA signature for lung squamous cell carcinoma

This study aimed to develop and assess a practical prognostic lncRNA signature for squamous cell carcinoma of the lung (LUSC).

Authors: Xiaoshun Shi, Fuxi Huang, Xiaobing Le, Xiaoxiang Li, Kailing Huang, Baoxin Liu, Viola Yingjun Luo, Yanhui Liu, Zhuolin Wu, Allen Menglin Chen, Ying Liang and Jiexia Zhang

Glyceraldehyde-3-phosphate dehydrogenase is overexpressed in colorectal cancer onset

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an essential regulator of glycolysis used as a housekeeping marker for gene/protein normalisation. Given the pivotal role of GAPDH in tumour metabolism, our ...

Authors: Míriam Tarrado-Castellarnau, Santiago Diaz-Moralli, Ibrahim H. Polat, Rebeca Sanz-Pamplona, Cristina Alenda, Víctor Moreno, Antoni Castells and Marta Cascante

Analytical validation of an mRNA-based urine test to predict the presence of high-grade prostate cancer

The challenge for prostate cancer (PCa) diagnosis is to improve the ability to distinguish indolent from aggressive PCa. Increased urinary levels of Distal-less Homeobox 1 (DLX1) and Homeobox C6 (HOXC6) mRNA have...

Authors: Daphne Hessels, Hans de Jong, Sander A. Jannink, Michael Carter, Manuel Krispin, Wim Van Criekinge, Leander Van Neste and Jack A. Schalken

Residual risk of transfusion-transmitted malaria infection in a malaria endemic sub-Saharan African setting

In Cameroon, as in many malaria endemic countries in Africa, blood donors are not routinely screened for Plasmodium infection that potentially could lead to severe malaria in some recipients. This study aimed at ...

Authors: Cécile Okalla Ebongue, Eveline Ngouadjeu Dongho, Gaétan Texier, Jean-Pierre Nda Mefo’o, Gerald Sume Etapelong, Lawrence Ayong and Carole Else Eboumbou Moukoko

Total lymphocyte count in cancer patients with lymphopenia treated with intravenous vitamin C: Results of an observational study

Lymphopenia commonly occurs in cancer patients and predicts poor prognosis. It is caused by radio- and chemotherapy, with malnutrition and treatment-related oxidative stress playing key roles in its pathogenes...

Authors: Dolores Margarita Rodríguez, Mery Elizabeth Guerrero, Bárbara Marisol Maldonado, Claudia Vollbracht and Sandra Aracely Herrera

Job performance in chronic hepatitis C virus patients treated with pegylated interferon-α2b plus ribavirin: an observational study

The employment status of workers with chronic hepatitis C has important implications for society and labor market, for organizations and for the individuals and their economic, social and psychological status....

Authors: Giulia Malaguarnera, Gaetano Bertino, Carmela Greco, Caterina Gagliano, Massimo Motta, Giuseppe Chisari, Venerando Rapisarda, Giuseppe Nunnari, Michele Vecchio, Filippo Drago and Michele Malaguarnera

Cryoinjury-induced acute myocardial infarction model and ameroid constrictor-induced ischemic heart disease model in adult micro-mini pigs for preclinical studies

Coronary artery diseases (CAD) are the most commonly occurring disorders in the developed countries. Development of new therapeutics and their success in clinical studies require confirmation of therapeutic ef...

Authors: Akinori Hirano, Jun Fujita, Hideaki Kanazawa, Shinji Kawaguchi, Noriko Handa, Yoshitake Yamada, Shigeo Okuda, Shuji Hishikawa, Takumi Teratani, Satoshi Kunita, Shugo Tohyama, Tomohisa Seki, Ryota Tabei, Kazuaki Nakajima, Yoshikazu Kishino, Marina Okada…

Comparative in vitro cytotoxic, anti-inflammatory and anti-microbiological activities of two indigenous Venda medicinal plants

The Vhembe region of the Limpopo province has a rich tradition of medicinal plants use. Traditionally, boiled roots of Ziziphus mucronata are used in the treatment of boils, general swelling and other skin infect...

Authors: M. T. Sigidi, C. P. Anokwuru, T. Zininga, M. P. Tshisikhawe, A. Shonhai, I. D. I. Ramaite, A. N. Traoré and N. Potgieter

Thymosin beta 4 treatment improves left ventricular function after myocardial infarction and is related to Up-regulation of chitinase 3-like-1 in mice

Thymosin beta 4 is a promising agent in preclinical regenerative and cardioprotection research. After myocardial injury it improves cell survival, reduces inflammation and activates epicardial progenitor cells...

Authors: Christoffer Stark, Mikko Helenius, Pekka Taimen, Rasmus Kentala, Antti Saraste, Tero-Pekka Alastalo, Timo Savunen and Juha Koskenvuo

Prognostic value of morphologic and morphometric analyses in IgA nephropathy biopsies

IgA nephropathy (IgAN) exhibits a variable course ranging from a benign condition to progressive renal failure with a high proportion of patients undergoing end-stage renal disease in the long term. It is uncl...

Authors: Patrizia Viola, Lucia Centurione, Paolo Felaco, Giuseppe Lattanzio, Tommaso D’Antuono, Marcella Liberatore, Roberta Di Pietro, Franco Oreste Ranelletti, Mario Bonomini and Francesca Bianca Aiello

A novel clinical approach to evaluating changes in fat oxidation in healthy, overnight-fasted subjects

Obesity is characterized by impaired fat oxidation, and, therefore, the development of therapeutics designed to enhance fat oxidation for treating obesity is a growing focus in drug discovery. Evaluating such ...

Authors: Stephanie A. Parsons, Karen P. Jones, Fanchao Yi, Christopher P. Bock, Christopher J. Petucci, Stella K. Betancourt, Stephen J. Gardell and Steven R. Smith

Optimization of CFTR-mRNA transfection in human nasal epithelial cells

Cystic fibrosis (CF) is the most common life-threatening inherited disease in the Caucasian population. It is caused by genetic defects in the cystic fibrosis transmembrane conductance regulator gene (CFTR), a cA...

Authors: Elena Fernández Fernández, Nadine Bangel-Ruland, Katja Tomczak and Wolf-Michael Weber

CD8⁺ tumor infiltrating lymphocytes strongly correlate with molecular subtype and clinico-pathological characteristics in breast cancer patients from Sudan

Breast Cancer in the Middle East and North Africa region (MENA) occurs in a younger age group of patients and is more aggressive than in Europe and North America. Little is known about the presence of immune i...

Authors: Mahmoud Mohamed, Hina Sarwath, Nada Salih, Devendra Bansal, Prem Chandra, Nazik E. Husain, Lotfi Chouchane, Ali A. Sultan and Shahinaz Bedri

Membrane cholesterol depletion reduces breast tumor cell migration by a mechanism that involves non-canonical Wnt signaling and IL-10 secretion

Migration and invasion are hallmarks of cancer cells that allow their dissemination to other tissues. Studying the cellular and molecular basis of cancer cell migration can help us to understand and control ca...

Authors: Fabiana Sélos Guerra, Luzia da Silva Sampaio, Sandra Konig, Martin Bonamino, Maria Isabel Doria Rossi, Manoel Luis Costa, Patricia Fernandes and Claudia Mermelstein

T-helper-1, T-helper-17, T-regulatory lymphocytes in hypertensive patients with diabetes mellitus type 2 or impaired glucose tolerance: association with clinical and metabolic parameters in a case control study

Low-grade inflammation plays significant role in the development of diabetes mellitus type 2 (DM2) and impaired glucose tolerance (IGT), but immune regulatory mechanisms involved in these processes are studied...

Authors: Irina V. Kologrivova, Tatiana E. Suslova, Olga A. Koshelskaya, Irina V. Vinnizkaya and Sergey V. Popov

Translational Medicine Communications: the home for sound translational science

Authors: Francesco Marincola and Philip Dooner